Document Type : Original Article
Authors
1
Department of Mechanical Engineering, Dez.C., Islamic Azad University, Dezful, Iran.
2
Department of Mechanical Engineering, Ahv.C., Islamic Azad University, Ahvaz, Iran.
3
Toxicology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4
1- Environmental Technologies Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishspur University of Medical Sciences, Ahvaz, Iran 2-Department of Environmental Health Engineering Ahvaz Jundishapur University of Medical Sciences,
10.22034/nawee.2026.590815.1249
Abstract
This experimental-intervention study was conducted at a pilot scale in 2022 to optimize the biogas production process from sugarcane vinasse specifically focusing on reducing the inhibitory effects of toxic compounds and optimizing operational parameters. The experiments were carried out based on the Biochemical Methane Potential (BMP) method at the Faculty of Agriculture, Ferdowsi University of Mashhad, Iran. The effect of six variables including temperature (37 and 55 °C), residence time (14 and 21 and 28 days), C/N ratio (1:20, 1:25, and 1:30), pH (6, 7, and 8), hydrogen peroxide concentration (2, 3 and 5 mg/L) and initial vinasse loading volume (5000 and 10000 ml) was investigated. Response surface methodology (RSM) and central composite design (CCD) were used to design the experiments. The amount of biogas produced in the process was measured based on the gas chromatography (GC) method. The results showed that the optimal conditions for biogas production were as follows: temperature 37 °C (mesophilic), time: 21 days, pH equal to 7, C/N ratio equal to one to 25, H2O2 amount equal to 3 g/L and loading volume 10000 ml. Under these conditions, the maximum biogas production efficiency reached 43.2%. Using a hydrogen peroxide concentration of 3 mg/L under these conditions helped to reduce the inhibitory effects of toxic compounds present in the vinasse and facilitated the biogas production process. These results indicate that the addition of hydrogen peroxide can be considered an effective strategy to reduce toxicity and increase the efficiency of the biogas production process.
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